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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
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Fecha : |
01/12/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T. |
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Afiliación : |
PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS G. CORBELLINI, Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; VANESSA B. LEOTTI, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; RODOLFO RIVERO, Laboratorio Regional Noroeste “Miguel C. Rubino”, División de Laboratorios Veterinarios “Miguel C. Rubino”, Paysandú, Uruguay; MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ M. VENZAL, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay. |
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Título : |
Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report) |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742 |
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DOI : |
10.1177/1040638720975742 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com |
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Contenido : |
Abstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. |
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Palabras claves : |
ANAPLASMA MARGINALE; BABESIA SPP; CATTLE; ENFERMEDADES DE LOS ANIMALES; MULTIPLEX PCR; PLATAFORMA DE SALUD ANIMAL; SENSITIVITY; SPECIFICITY; TICK-BORNE DISEASES. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 02587naa a2200337 a 4500
001 1061695
005 2021-05-21
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638720975742$2DOI
100 1 $aPARODI, P.
245 $aValidation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report)$h[electronic resource]
260 $c2021
500 $aArticle history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com
520 $aAbstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.
653 $aANAPLASMA MARGINALE
653 $aBABESIA SPP
653 $aCATTLE
653 $aENFERMEDADES DE LOS ANIMALES
653 $aMULTIPLEX PCR
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSENSITIVITY
653 $aSPECIFICITY
653 $aTICK-BORNE DISEASES
700 1 $aCORBELLINI, L.G.
700 1 $aLEOTTI, V.B.
700 1 $aRIVERO, R.
700 1 $aMIRABALLES, C.
700 1 $aRIET-CORREA, F.
700 1 $aVENZAL, J.M.
700 1 $aARMÚA-FERNÁNDEZ, M.T.
773 $tJournal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
17/05/2021 |
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Actualizado : |
18/05/2021 |
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Tipo de producción científica : |
Abstracts/Resúmenes |
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Autor : |
CIGANDA, V.; GRAHMANN, K.; QUINCKE, A. |
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Afiliación : |
VERONICA SOLANGE CIGANDA BRASCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; KATHRIN GRAHMANN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; JUAN ANDRES QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Pérdidas de nitrógeno por lixiviación de nitratos en sistemas de cultivo continuo y rotación cultivo-pastura. |
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Complemento del título : |
Resumen: 638. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
In: Congreso Latinoamericano de Ciencias de Suelo, 22.; 2do Congreso Uruguayo de Suelos, 2; Encuentro de la SUCS, 10. Diversidad Productiva:
pilar del manejo sostenible de los suelos. Montevideo, Uruguay: SUCS; SLSC, 2019. |
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Idioma : |
Español |
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Palabras claves : |
EXPERIMENTO A LARGO PLAZO; LIXIVIACIÓN. |
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Thesagro : |
CULTIVOS; NUTRIENTES; PASTURAS; SISTEMAS DE PRODUCCION; SUELOS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15612/1/Congreso-Latinoamericano-de-Ciencia-del-Suelo-22.-Montevideo.2019-Ciganda.Resumen.pdf
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Marc : |
LEADER 00808nam a2200205 a 4500
001 1062082
005 2021-05-18
008 2019 bl uuuu u01u1 u #d
100 1 $aCIGANDA, V.
245 $aPérdidas de nitrógeno por lixiviación de nitratos en sistemas de cultivo continuo y rotación cultivo-pastura.$h[electronic resource]
260 $aIn: Congreso Latinoamericano de Ciencias de Suelo, 22.; 2do Congreso Uruguayo de Suelos, 2; Encuentro de la SUCS, 10. Diversidad Productiva: pilar del manejo sostenible de los suelos. Montevideo, Uruguay: SUCS; SLSC$c2019
650 $aCULTIVOS
650 $aNUTRIENTES
650 $aPASTURAS
650 $aSISTEMAS DE PRODUCCION
650 $aSUELOS
653 $aEXPERIMENTO A LARGO PLAZO
653 $aLIXIVIACIÓN
700 1 $aGRAHMANN, K.
700 1 $aQUINCKE, A.
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